Modulation of rat erythrocyte antioxidant defense system by buthionine sulfoximine and its reversal by glutathione monoester therapy
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چکیده
منابع مشابه
Glutathione depletion by L-buthionine sulfoximine antagonizes taxol cytotoxicity.
Taxol is a naturally occurring chemotherapeutic agent that is active against a variety of tumors. Taxol is believed to act by binding tightly to microtubules and preventing their disaggregation. Others have shown that depletion of cellular glutathione results in the disaggregation of microtubules, presumably by allowing the oxidation of some or all of the cysteine residues in tubulins. We studi...
متن کاملGlutathione depletion by buthionine sulfoximine induces DNA deletions in mice.
Oxidative stress and genomic rearrangements play a role in cancer development. l-Buthionine-sulfoximine (BSO) induces oxidative stress in a cell by irreversibly inhibiting gamma-glutamylcysteine synthetase, an essential enzyme for the synthesis of glutathione (GSH). We postulated that oxidative stress induced by GSH depletion might lead to genomic rearrangements, such as DNA deletions, and that...
متن کاملPotent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine).
Buthionine sulfoximine (S-n-butyl homocysteine sulfoximine), the most potent of a series of analogs of methionine sulfoximine thus far studied (Griffith, O.W., Anderson, M.E., and Meister, A. (1979) J. Biol. Chem. 254, 1205-1210), inhibited gamma-glutamylcysteine synthetase about 20 times more effectively than did prothionine sulfoximine and at least 100 times more effectively than methionine s...
متن کاملGlutathione turnover in human erythrocytes. Inhibition by buthionine sulfoximine and incorporation of glycine by exchange.
Previous investigations have indicated that [`4C]glycine is incorporated into erythrocyte glutathione at a rate which exceeds by severalfold the rate of cysteine and glutamate incorporation. The discrepancy between the rates has generally been attributed to the known differences in the rates at which these amino acids are transported into erythrocytes. In the present investigation, human erythr...
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We have measured glutathione content in small tissue samples derived from biopsies of primary and metastatic human colon tumors and from colon cancer cell lines in tissue culture and xenografts in athymic mice. Measurements were performed using an enzymatic cycling assay designed to quantitate extremely low levels of glutathione (GSH) (down to 10(-14) mol) from perchlorate extracts of tissue sa...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
سال: 2004
ISSN: 0925-4439
DOI: 10.1016/j.bbadis.2003.11.004